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Cell functions - Membrane-located activities - Photosynthesis - Light and photosynthetic pigments - Thin-Layer Chromatography
Thin-Layer Chromatography
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| Description |
Thin-layer chromatography allows molecules to be segregated according to size.
To accomplish this, a plate of glass is covered with a thin layer of cellulose matrix. The matrix molecules form a close network. If the glass plate is suspended vertically in a special solvent, the solution rises up the plate and through the matrix by capillary action.
If a mixture of substances, e.g. pigments, is applied to the bottom edge of the plate, they are carried along by the solvent. In doing so, smaller molecules migrate faster through the matrix than large ones. In this way, the mixture is separated into its constituents.
Duration: 00:39 min
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Sources
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The principle of thin-layer chromatography. 2D animation. IWF, Göttingen, 1999.
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Abbreviations
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Order |
This video is part of the CD-ROM The Cell I - Life from Light and Air. You can order it at the IWF Knowledge and Media gGmbH.
Also available as clip.
Order no.:
C 7190
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